What is the primary purpose of keeping doors and windows?
What is the primary purpose of keeping doors and windows closed during the laboratory session? To reduce the incidence of contamination from microbes traveling in the air. After using an inoculating loop or needle to transfer a culture from one medium to the next, what stop should be taken next to avoid contamination.
Which steps are necessary for cleaning a spill involving broken glass quizlet?
Soak the spill with disinfectant for at least 20 minutes, then dispose of the broken glass in a designated container for broken glass .
What is the procedure to clean up a culture spill on your benchtop?
If you spill or drop a culture , after notifying the instructor, place a paper towel over the spill and pour disinfectant on the towel. Wait for 15 minutes then clean the spill with fresh paper towels. Remember to place paper towels in wastebaskets and wash your hands carefully. Dispose of any broken glass properly.
What is the #1 best safety procedure in regard to microbes?
Never eat or drink in the laboratory while working with microorganisms. Keep your fingers out of your mouth, and wash your hands before and after the laboratory activity. Cover any cuts on your hands with a bandage. Gloves may be worn as extra protection.
What should you always do at the end of a lab?
What should you always do at the end of the lab ? clean up.
What is lab safety and why is it important?
Lab safety protects property. Chemicals that are improperly stored or handled can react with one another to cause fires and explosions. The subsequent property damage can affect the lab , all the work performed there, and all the people depending on what that lab does.
What is the importance of cleaning workbench before and after use?
Just as it is vital to clean your kitchen after cooking a meal, laboratory workbenches need to be cleaned between uses. Keeping your lab bench clean ensures leftover dirt or other materials do not contaminate future projects.
Which steps are necessary for cleaning a spill involving broken glass?
Using tongs or forceps, place broken glass /sharps in sharps container. Use dust pan and broom for shattered glass . Cover the spill area with absorbent materials such as Green-Z, paper towels, SSS clean -up powder. Remove the absorbent material.
What is the last step of any spill cleanup procedure quizlet?
What is the last step of any spill cleanup procedure ? Wipe down the affected area with detergent and water.
Why is it important to wash your hands and wipe your work area clean before you work with microorganisms?
Clean things: hands can pick up dust-borne and water-borne microorganisms in the environment. Scratch or wipe your body: introduce microorganisms into cuts or pick up some of the many microorganisms that inhabit your skin and body cavities. Shake other people’s hands : exchange germs with other people.
What should be used to clean up a bacterial spill?
Remove contaminated lab coat or clothing and wash exposed skin. Put on clean gloves and lab coat. Prepare enough volume of a 1:10 dilution of chlorine bleach or other approved disinfectant to saturate the contaminated area. Contain the spill with paper towels or other absorbent material such as bench liners.
How do bacteria grow in a lab?
Once the culture dish is prepared, use a sterile cotton swab or inoculating needle and swab the inside of your cheek. Very gently rub the swab over the agar in a few zigzag strokes and replace the lid on the dish. You’ll need to let the dish sit in a warm area for 3-7 days before bacteria growth appears.
What are five safety factors to consider when working with bacteria?
Nine Safe Practices for the Microbiology Laboratory Treat all microorganisms as potential pathogens. Sterilize equipment and materials. Disinfect work areas before and after use. Wash your hands. Never pipette by mouth. Do not eat or drink in the lab, nor store food in areas where microorganisms are stored. Label everything clearly.
How do you kill bacteria in a petri dish?
Before disposing of dishes in the trash the bacteria should be destroyed. Pour a small amount of household bleach over the colonies while holding dish over sink. Caution – do not allow bleach to touch your skin, eyes or clothes. It will burn!
What are the risks of growing bacteria?
Culturing microorganisms can lead to the growth of dangerous pathogens. Pathogens may enter the human body through skin, eyes, puncture wounds, inhalation, or ingestion. Students who have compromised immune systems should consult the teacher or their doctor before participating in microbiology experiments.